Since the discovery and development of proteome, methods used in quantifying proteomics has been constantly improving. The most common methods of quantifying proteomics are including DIGE (difference gel electrophoresis), iCAT(Isotope-coded affinity tag) and SILAC(Stable Isotope Labeling with Amino Acids in Cell Cultures). In 2004, iTRAQ (isobaric tags for relative and absolute quantitation) was developed by Applied Biosystems Incorporation—ABI. After that, iCAT and iTRAQ became the most powerful approaches in this aspect. This post mainly focuses on introducing iTRAQ.
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