Gel Electrophoresis Used For RNA Gel Analysis
31318 10/18/2014 Health And Fitness 307 /Gel electrophoresis is a method of separating (based on size, electric charge, and other physical properties) macromolecules such as nucleic acids and proteins. The term electrophoresis is used to describe the migration of a charged particle under the influence of an electric field. Therefore what gel electrophoresis is a technique in which the molecules are forced through the gel located in a space between two boundaries (edges), driven by an electric current is concerned. At each of the ends of the activated gel electrodes is providing no attractive force. Thus properties of the molecule especially possessing ionizable group as quickly determine an electric field can move the molecule through a gelatinous medium.
An important application is gel electrophoresis DNA technology.
Gel electrophoresis is one of the most important tools in molecular biology and is of critical value in many aspects of handling and genetic study. One use is the identification of individual DNA molecules in band patterns that they produce in the gel after electrophoresis was der cut with several restriction enzymes. Viral DNA, plasmid DNA and chromosomal particular segments can be identified by this technique. Another use is the separation and purification of individual fragments containing interesting genes, which can be recovered from the gel with full biological activity.
Gel electrophoresis makes it possible to determine the genetic difference and the evolutionary relationship between plant and animal species. Using this technology it is possible to identify the proteins separated and differing in at least one amino acid.
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